Sublingual protein delivery by a mucoadhesive patch made of natural polymers

The sublingual mucosa is an appealing route for drug administration. However, in the context of increased use therapeutic proteins, development protein delivery systems that will protect bioactivity needed. As proteins are fragile and complex molecules, current formulations liquid dosage. Yet, dilution short residence time at main barriers control dose delivered. In this work, a simple scaffold based on assembly two polysaccharides, chitosan hyaluronic acid, presented. natural polymers were assembled by Layer-by-Layer methodology to produce mucoadhesive oro-dispersible freestanding membrane, shown be innocuous epithelial human cells. functionalization membrane with led production bioactive patch efficient loading release suitable mechanical properties manipulation. Sublingual administration mouse evidenced absence inflammation extended contact between model ovalbumin compared formulation. fluorescent demonstrated penetration epithelium 10 min after Moreover, migration assay chemokine incorporated into showed no decrease loaded enzymatic release. This study therefore provides promising strategy develop system. Although oral largely used delivery, it has limitations can degraded pH or gastric enzymes. appears as interesting approach presented polysaccharides patch. produced allowed well protection their bioactivity. An formulation was highlighted model.